Product Principles

Bioluminescence Imaging (BLI): BLI is a technology that uses light emitted by enzyme-catalyzed reactions to report activity at the molecular level, which does not need the excitation light source, the background is low and the signal-to-noise ratio is high.

Fluorescence Imaging (FLI): FLI is the visualization of fluorescent dyes or proteins as labels for molecular processes or structures, which needs the excitation light source, the background is relatively high and the signal-to-noise ratio is relatively low.

Cherenkov luminescence imaging (CLI): CLI is an emerging imaging modality, similar to bioluminescence imaging, that captures visible photons emitted by Cherenkov radiation. It basically is the optical imaging of radiotracers that emit charged particles traveling faster than the phase velocity of light in that particular medium.

X-ray Imaging: A beam of X-rays is produced by an X-ray generator and is projected toward the object. A certain amount of the X-rays or other radiation is absorbed by the object, dependent on the object's density and structural composition. The X-rays that pass through the object are captured behind the object by a detector.

Brief Introduction

Engineered with superior optics and industry leading technology, Spectral Instruments Imaging provides unrivaled sensitivity for bioluminescence, fluorescence, and X-ray in vivo imaging. Intelligently designed around the needs of animal scientists, all Spectral Instrument Imaging systems include a robust build, patented LED illumination source, custom filter options, -90℃ cooled camera, and absolute calibration. Lago and Ami systems enable high throughput imaging and field upgradable X-ray.

Product Characteristics

The flagship product Lago X has below characteristics:

1、-90°C Absolute Cooled Camera with Grade One Plus CCD

Ultra cold for maximum sensitivity

2、Unprecedented Detection

Previously unattained sensitivity for Bioluminescence and Fluorescence (BLI/FLI)

3、Unrivaled Sensitivity

Minimum Detectable Radiance is 45 photons/sec/cm^2/sr

4、LED Fluorescence Illumination

Cutting edge patented LED based illumination

5、14 Wavelengths/20 Filters

Be prepared for any fluorophore at any time. No need to change filters.

6、Robust Design

Built to perform for the crucial demands of imaging cores and CRO's

7、Absolute Calibration

Ensures quantifiable data

8、Field Upgradable

Lago units are field upgradable to include X-ray, Access Ports and Quantitative 3D Optical Imaging, with minimal downtime.

9、10 Mouse Capacity (BLI/FLI/X-ray)

Industry leading 25cm x 25cm field of view

10、Software is 100% License Free

Available for PC & Mac users

Application Fields

Using bioluminescent or fluorescent labeling technology to label a variety of research objects, such as tumor cells, immune cells, stem cells, genes, bacteria, viruses, peptides, antibodies, nanomaterials, drugs, etc. Widely used in small animal models’ imaging based on cancer, stem cells, infection, inflammation, immune diseases, neurological diseases, cardiovascular diseases, metabolic diseases, gene therapy and other diseases to do the preclinical research on molecular mechanism and related drug research and development.

1、Bioluminescence vs. MRI and µCT

• Orthotopic lung carcinoma model in mice

• Athymic Nude-Foxn1nu mice, females, 10-11 weeks old

• Challenge:  Human Lung Carcinoma A549 cells encoding firefly luciferase (Fluc), 5.74 x 105 cells/0.1 mL 1xPBS, IV

• Fast, real-time data acquisition: Requires only seconds to acquire data. Faster than PET, SPECT, and MRI

2、Low Cell Copy Number Tracking

• Mice were sub-lethally irradiated, and then

• Challenged with DiR-labeled T-cells (by tail-vein IV injection) to evaluate T-cell presentation at peripheral lymph nodes.

• T-cell presentation of peripheral lymph nodes

3、Specificity of Therapeutic Targeting In Vivo

Ref: PDGFRβ-specific affibody-directed delivery of a photosensitizer, IR700, is efficient for vascular-targeted photodynamic therapy of colorectal cancer.

4、In vivo, Real Time Tracking of NPs across BBB

Model:

• Orthotopic Glioblastoma multiforme murie xenograft model

• 5 x 105 U87M2/luc human GBM cell line injected into brain parenchyma w/ stereotaxic frame

• 4 nmol IR780-phospholipid micelle NP, in 100 uL PBS, injected IV via tail vein

• IR780-phospholipid micelle tracked in vivo by NIRF imaging (Ex/Em 745/810 nm, 1 sec)

• Orthotopic location of U87M2/luc cells confirmed by BLI

Ref: Near infrared fluorescent imaging of brain tumor with IR780 dye incorporated phospholipid nanoparticles.

5、Evaluation of Glioma Treatment Efficacy

Proposed Therapeutic Model: 

• Given an establish lower grade glioma

• Take biopsy, run qPCR to genotype tumor as IDH1 +/-

• If IDH1 +, treat with GMX-1778 loaded microparticles

Experiment Design: 

• Establish lower grade glioma in SCID mouse model with either IDH1+ (MGG152) or IDH1- (wild type, U87) glioma cell line, both transduced to express luciferase

• At day 15 post-challenge, treat with 4mm diameter PLGA microparticles (MPs), containing GMX-1778, at 5µM, designed to provide sustained, local delivery.

Ref: Genotype-targeted local therapy of glioma

6、In Vivo Detection of Metastatic Tumors

• Model:  Orthotopic breast cancer in mice.

• Challenge:  106 4T1 breast tumor cells, injected into mouse mammary fat pad

• Result: The tumor shrinks under treatment of Dox+Th1 and Dox+Th17

Ref：Doxorubicin Eliminates Myeloid-Derived Suppressor Cells and Enhances the Efficacy of Adoptive T-cell Transfer in Breast Cancer

7、Coregistration

SII can enable coregistration with μCT, PET & SPECT